2016 Thai Football Division 3 Tournament Eastern Region is the 1st season of the League competition since its establishment in 2016. It is in the fourth tier of the Thai football league system.
All matches played in Chonburi
| Section | Club | Province | Years | |
|---|---|---|---|---|
| 1 | Chonburi | 2016 | ||
| 1 | Navanakorn | Pathum Thani | 2016 | |
| 1 | Assumption College Sriracha | Chonburi | 2016 | |
| 2 | Na-Me United | Bangkok | 2016 | |
| 2 | Siriroj | Chonburi | 2016 | |
| 2 | Khaobaisri | Rayong | 2016 | |
| 3 | Traf Maptaphut | Rayong | 2016 | |
| 3 | Bankhai United | Rayong | 2016 | |
| 3 | Takhiantia | Chonburi | 2016 | |
| 4 | Hakka Association of Thailand | Bangkok | 2016 | |
| 4 | Kohkwang Subdistrict Municipality | Chanthaburi | 2016 | |
| 4 | Pattana | Chonburi | 2016 | |
| 5 | Rayong City | Rayong | 2016 | |
| 5 | Isan Pattaya | Chonburi | 2016 | |
| 5 | Banbung Rovers | Chonburi | 2016 | |
| 6 | Sansuk Municipality Meangkanyang | Chonburi | 2016 | |
| 6 | Pak Chong City | Nakhon Ratchasima | 2016 | |
| 6 | Bangpakong Sports Association | Chachoengsao | 2016 | |
| 7 | Institute of Physical Education Chonburi | Chonburi | 2016 | |
| 7 | Pattaya City Club | Chonburi | 2016 | |
| 7 | Salaeng Subdistrict Municipality | Chanthaburi | 2016 |
Group stage: A total 21 clubs will be divided into four groups of three clubs except group 7 which has four clubs to play round-robin matches at a neutral venue. The best clubs of group A and G qualify to Quarter-finals round of the knock-out stage. The best two clubs of group B, C, D, E, F and runner-up of group A and G will qualify to Second round of the knock-out stage.
Knock-out stage: A total of 14 clubs which has qualified from the group stage will play single-elimination stage until there are only two finalists of the tournament.