The men's 400 metres at the 2016 IPC Athletics European Championships was held at the Stadio Olimpico Carlo Zecchini in Grosseto from 11–16 June.
| T12[1] | Joan Munar Martinez | 50.11 CR | Jose Luis Fernandez Taular | 50.87 PB | |||
| T13[2] | Egor Sharov | 48.37 ER | Aleksei Labzin | 50.16 SB | Hakan Cira | 51.03 PB | |
| T20[3] | Rodrigue Massianga | 48.65 CR | Artem Muratov | 48.93 | Maksim Ovcharenko | 48.95 PB | |
| T34[4] | Henry Manni | 52.46 CR | Isaac Towers | 54.22 | Ben Rowlings | 54.41 SB | |
| T36[5] | Evgenii Shvetcov | 55.47 | Krzysztof Ciuksza | 56.48 PB | Artem Arefyev | 57.62 | |
| T38[6] | Andrei Vdovin | 50.52 WR | Chermen Kobesov | 51.83 | Lorenzo Albaladejo Martinez | 55.35 | |
| T44[7] | Johannes Floors (T43) | 48.67 CR | David Behre (T43) | 49.71 SB | Emanuele di Marino | 55.83 SB | |
| T47[8] | Aleksei Kotlov | 49.81 CR | Antonis Aresti | 50.44 | Alexander Pototschnig | 52.93 PB | |
| T51[9] | Peter Genyn | 1:18.09 WR | Alvise de Vidi | 1:24.48 SB | Toni Piispanen | 1:25.98 SB | |
| T52[10] | Thomas Geierspichler | 1:01.61 CR | Beat Bösch | 1:03.50 SB | Mário Trindade | 1:07.12 SB | |
| T53[11] | Vitalii Gritsenko | 49.01 CR | Moatez Jomni | 49.40 PB | Mickey Bushell | 51.04 SB | |
| T54[12] | David Weir | 46.10 CR | Richard Chiassaro | 46.46 PB | Aleksei Bychenok | 46.62 | |