The 2016 Challenger La Manche was a professional tennis tournament played on indoor hard courts. It was the 23rd edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Cherbourg, France between 22 and 28 February.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Serbia | Filip Krajinović | 98 | 1 | |
 Japan | Yoshihito Nishioka | 110 | 2 | |
 France | Pierre-Hugues Herbert | 111 | 3 | |
| Daniel Brands | 132 | 4 | ||
 Czech Republic | Adam Pavlásek | 144 | 5 | |
 Australia | Jordan Thompson | 149 | 6 | |
 Russian Federation | Karen Khachanov | 150 | 7 | |
| France | Kenny de Schepper | 152 | 8 |
The following players received wildcards into the singles main draw:
The following player received a special exemption into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2016 Challenger La Manche – Singles.
Jordan Thompson def. Adam Pavlásek 4–6, 6–4, 6–1See main article: 2016 Challenger La Manche – Doubles.
Ken Skupski / Neal Skupski def. Yoshihito Nishioka / 
Aldin Šetkić 4–6, 6–3, [10–6]