The 2016 Brasil Open was a tennis tournament played on outdoor clay courts. It was the 16th edition of the Brasil Open, and part of the ATP World Tour 250 series of the 2016 ATP World Tour. It took place from February 22 through February 28, 2016, in São Paulo, Brazil. Third-seeded Pablo Cuevas won the singles title.
See main article: 2016 Brasil Open – Singles.
Pablo Carreño Busta 7–6(7–4), 6–3See main article: 2016 Brasil Open – Doubles.
Horacio Zeballos defeated Pablo Carreño Busta / David Marrero 4–6, 6–1, [10–5]| Country | Player | Ranking1 | Seed | |
|---|---|---|---|---|
 France | Benoît Paire | 22 | 1 | |
 Brazil | Thomaz Bellucci | 32 | 2 | |
| Pablo Cuevas | 45 | 3 | ||
| Argentina | Federico Delbonis | 46 | 4 | |
| Spain | Albert Ramos Viñolas | 47 | 5 | |
 Italy | Paolo Lorenzi | 50 | 6 | |
| Spain | Nicolás Almagro | 53 | 7 | |
| Spain | Pablo Andújar | 62 | 8 |
The following players received wildcards into the main draw:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
Fabio Fognini (abdominal strain) → replaced by Daniel Gimeno Traver Guido Pella (non medical)→replaced by Luca Vanni Pablo Andújar (right elbow injury) Horacio Zeballos (dehydration)| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Brazil | Marcelo Melo | Brazil | Bruno Soares | 11 | 1 | |
| Spain | ||||||
| Argentina | Máximo González | Brazil | André Sá | 104 | 3 | |
| Nicholas Monroe |  Austria | Philipp Oswald | 115 | 4 |
The following pairs received wildcards into the main draw:
Nicolás Almagro / Eduardo Russi Assumpção Rogério Dutra Silva / João SouzaThe following pair received entry as alternates:
Pedro Bernardi / Guilherme Clezar Marcel Granollers (lower back injury)