| Champ: |  Dylan Alcott |
| Runner: |  David Wagner |
| Score: | 6–2, 6–2 |
| Before Name: | Australian Open – Wheelchair quad singles |
| After Name: | Australian Open – Wheelchair quad singles |
See main article: 2016 Australian Open.
Defending champion Dylan Alcott defeated David Wagner in the final, 6–2, 6–2 to win the quad singles wheelchair tennis title at the 2016 Australian Open.
Standings are determined by: 1. number of wins; 2. number of matches; 3. in two-players-ties, head-to-head records; 4. in three-players-ties, percentage of sets won, or of games won; 5. steering-committee decision.