| Date: | 30 May–5 June |
| Edition: | 2nd |
| Category: | ITF Women's Circuit |
| Location: | Eastbourne, United Kingdom |
| Prize Money: | $50,000 |
| Champs: |  Alison Riske |
| Champd: |  Yang Zhaoxuan / Zhang Kailin |
| After Year: | no |
The 2016 Aegon Eastbourne Trophy was a professional tennis tournament played on outdoor grass courts. It was the second edition of the tournament and part of the 2016 ITF Women's Circuit, offering a total of $50,000 in prize money. It took place in Eastbourne, United Kingdom, on 30 May–5 June 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| United States | Alison Riske | 93 | 1 | |
| Donna Vekić | 96 | 2 | ||
| China | Zhang Kailin | 117 | 3 | |
 Austria | Tamira Paszek | 123 | 4 | |
| China | Duan Yingying | 133 | 5 | |
 Poland | Urszula Radwańska | 140 | 6 | |
| China | Wang Yafan | 143 | 7 | |
 France | Océane Dodin | 148 | 8 |
The following player received a wildcard into the singles main draw:
The following players received entry from the qualifying draw:
The following player received entry by a lucky loser spot:
The following players received entry by protected rankings:
See main article: 2016 Aegon Eastbourne Trophy – Singles.
Alison Riske def. 
Tara Moore, 4–6, 7–6(7–5), 6–3See main article: 2016 Aegon Eastbourne Trophy – Doubles.
Yang Zhaoxuan / Zhang Kailin def. Asia Muhammad / Maria Sanchez, 7–6(7–1), 6–1