| Champ: |  Simona Halep |
| Runner: | Angelique Kerber |
| Score: | 6–2, 6–3 |
| Draw: | 56 |
| Seeds: | 16 |
| Type: | singles doubles |
| Before Name: | Qatar Total Open – Singles |
| After Name: | Qatar Total Open – Singles |
See main article: 2014 Qatar Total Open.
Simona Halep defeated Angelique Kerber in the final, 6–2, 6–3 to win the singles tennis title at the 2014 WTA Qatar Open.
Victoria Azarenka was the two-time reigning champion, but withdrew before the tournament due to a foot injury.
The top eight seeds receive a bye into the second round.
See also: 1 and 1. 
Li Na (third round)
See also: 2 and 4. 
Agnieszka Radwańska (semifinals)
See also: 3 and 2. 
Petra Kvitová (quarterfinals)
See also: 4 and 3. 
Sara Errani (quarterfinals)
See also: 5 and 2. 
Jelena Janković (semifinals)
See also: 6 and 1. Angelique Kerber (final)
See also: 7 and 3. Simona Halep (champion)
See also: 8 and 4. Caroline Wozniacki (second round)
See also: 9 and 1. Ana Ivanovic (second round)
See also: 10 and 2. 
Dominika Cibulková (first round, retired because of a gastrointestinal illness)
See also: 11 and 3. Roberta Vinci (first round)
See also: 12 and 4. 
Samantha Stosur (second round)
See also: 13. 
Carla Suárez Navarro (withdrew because of an elbow injury)
See also: 14 and 1. 
Sloane Stephens (first round)
See also: 15 and 3. 
Eugenie Bouchard (first round)
See also: 16 and 2. 
Kirsten Flipkens (first round)