The 2014 Advantage Cars Prague Open, also known as Advantage Cars Prague Open by I. ČLTK Praha for sponsorship reasons, was a professional tennis tournament played on clay courts. It was the 21st edition of the tournament which was part of the 2014 ATP Challenger Tour. It took place in Prague, Czech Republic between 4 and 10 August 2014.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Argentina | Diego Schwartzman | 100 | 1 | |
 Russian Federation | Andrey Kuznetsov | 107 | 2 | |
 Poland | Michał Przysiężny | 145 | 3 | |
 Austria | Gerald Melzer | 147 | 4 | |
 France | Grégoire Burquier | 171 | 5 | |
 Slovakia | Miloslav Mečíř | 173 | 6 | |
| Russian Federation | Valery Rudnev | 211 | 7 | |
 Ireland | Louk Sorensen | 212 | 8 |
The following players received wildcards into the singles main draw:
Dušan Lojda Adrian Sikora David Simunek Robin StanekThe following player received a special exemption into the singles main draw:
The following player entered into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
Tomáš PapikSee main article: 2014 Advantage Cars Prague Open – Singles.
Diego Schwartzman def. 
André Ghem 6–4, 7–5See main article: 2014 Advantage Cars Prague Open – Doubles.
Andrey Kuznetsov def. 
Roberto Maytín / 
Miguel Ángel Reyes-Varela 7–5, 7–5
