The 2013 Guimarães Open was a professional tennis tournament played on hard courts. It was the first edition of the tournament which was part of the 2013 ATP Challenger Tour. It took place in Guimarães, Portugal between 22 and 28 July 2013.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| João Sousa | 93 | 1 | ||
| Benjamin Becker | 99 | 2 | ||
 Japan | Go Soeda | 109 | 3 | |
 Romania | Marius Copil | 136 | 4 | |
 Spain | Daniel Muñoz de la Nava | 145 | 5 | |
 Italy | Matteo Viola | 147 | 6 | |
| Italy | Flavio Cipolla | 150 | 7 | |
 France | Josselin Ouanna | 160 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Iván Arenas-Gualda Andrés Artuñedo Matteo Donati Jaime Pulgar-GarcíaSee main article: 2013 Guimarães Open – Singles.
Marius Copil 6–3, 6–0See main article: 2013 Guimarães Open – Doubles.
James Cluskey / 
Maximilian Neuchrist vs. Roberto Ortega-Olmedo / Ricardo Villacorta-Alonso 6–7(5–7), 6–2, [10–8]