| Date: | 27 September – 3 October |
| Edition: | 4th |
| Location: | Naples, Italy |
| Champs: |  Fabio Fognini |
| Champd: |  Daniel Muñoz-de la Nava / Simone Vagnozzi |
The 2010 Tennislife Cup was a professional tennis tournament played on outdoor red clay courts. It was the fourth edition of the tournament which was part of the 2010 ATP Challenger Tour. It took place in Naples, Italy between 27 September and 3 October 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
| Italy | Potito Starace | 50 | 1 | |
| Italy | Fabio Fognini | 68 | 2 | |
| Frederico Gil | 84 | 3 | ||
| Italy | Filippo Volandri | 97 | 4 | |
| Spain | ||||
| Spain | Albert Ramos-Viñolas | 110 | 6 | |
| Italy | Paolo Lorenzi | 111 | 7 | |
| Italy | Simone Bolelli | 116 | 8 |
The following players received wildcards into the singles main draw:
Alberto Brizzi Enrico Fioravante Fabio Fognini Potito StaraceThe following players received entry from the qualifying draw:
Andrea Arnaboldi (as a Lucky Loser)
Farrukh Dustov
Boris Pašanski
Aldin Šetkić Luca VanniSee main article: 2010 Tennislife Cup – Singles. Fabio Fognini def. Boris Pašanski, 6–4, 4–2, RET.
See main article: 2010 Tennislife Cup – Doubles. Daniel Muñoz-de la Nava / Simone Vagnozzi def. 
Andreas Haider-Maurer / Bastian Knittel, 1–6, 7–6(5), [10–6]