See main article: 2010 Heineken Open.
Juan Martín del Potro was the defending champion, but chose not to compete.
John Isner won in the final, 6–3, 5–7, 7–6(7–2), against Arnaud Clément.
The top four seeds receive a bye to the second round.
All seeded players received a bye into the second round, and all players playing in the fourth qualifier received a bye into the second round.