| Year: | 2010 |
| Team: | Cal Poly Mustangs |
| Sport: | football |
| Conference: | Great West Conference |
| Short Conf: | GWC |
| Record: | 7–4 |
| Conf Record: | 2–2 |
| Head Coach: | Tim Walsh |
| Hc Year: | 2nd |
| Cooff Coach1: | Brian Cook |
| Cooc1 Year: | 2nd |
| Cooff Coach2: | Saga Tuitele |
| Cooc2 Year: | 2nd |
| Off Scheme: | Triple option |
| Def Coach: | Greg Lupfer |
| Dc Year: | 2nd |
| Def Scheme: | 3–4 |
| Stadium: | Alex G. Spanos Stadium |
The 2010 Cal Poly Mustangs football team represented California Polytechnic State University, San Luis Obispo as member of the Great West Conference (GWC) during the 2010 NCAA Division I FCS football season. Led by second-year head coach Tim Walsh, Cal Poly compiled an overall record of 7–4 with a mark of 2–2 in conference play, placing third in the GWC. The Mustangs played home games at Alex G. Spanos Stadium in San Luis Obispo, California.
See also: 2010 Humboldt State Lumberjacks football team.
See also: 2010 Montana Grizzlies football team.
See also: 2010 Texas State Bobcats football team.
See also: 2010 McNeese State Cowboys football team.
See also: 2010 Fresno State Bulldogs football team. Fresno State outscored Cal Poly 17-0 in the second half in the first meeting between the two rivals since 1985. Bulldogs go to 8-0 vs. FCS/1-AA teams under Pat Hill.
See also: 2010 Old Dominion Monarchs football team.
See also: 2010 Saint Francis Red Flash football team.
See also: 2010 UC Davis Aggies football team.
See also: Battle for the Golden Horseshoe.