The 2009 Status Athens Open was a professional tennis tournament played on outdoor clay courts. It was part of the 2009 ATP Challenger Tour. It took place in Athens, Greece between 6 and 12 April 2009.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Czech Republic | Ivo Minář | 96 | 1 | |
 Uzbekistan | Denis Istomin | 106 | 2 | |
 Romania | Victor Crivoi | 115 | 3 | |
| Roko Karanušić | 116 | 4 | ||
| Czech Republic | Jiří Vaněk | 122 | 5 | |
| Pablo Cuevas | 129 | 6 | ||
 Serbia | Ilija Bozoljac | 133 | 7 | |
| Czech Republic | Lukáš Rosol | 137 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2009 Status Athens Open – Singles. Rui Machado def. 
Daniel Muñoz de la Nava, 6–3, 7–6(4)
See main article: 2009 Status Athens Open – Doubles. 
Rameez Junaid / Philipp Marx def. Jesse Huta Galung / Rui Machado, 6–4, 6–3
