The 2009 San Luis Potosí Challenger was a professional tennis tournament played on outdoor red clay courts. It was part of the 2009 ATP Challenger Tour. It took place in San Luis Potosí, Mexico between 6 and 12 April 2009.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Argentina | Horacio Zeballos | 126 | 1 | |
 Colombia | Santiago Giraldo | 146 | 2 | |
 Brazil | Franco Ferreiro | 155 | 3 | |
| Argentina | Mariano Puerta | 205 | 4 | |
| Colombia | Alejandro Falla | 207 | 5 | |
| Michael Lammer | 219 | 6 | ||
 Mexico | Santiago González | 223 | 7 | |
 Italy | Paolo Lorenzi | 227 | 8 |
The following players received wildcards into the singles main draw:
Luis Manuel Flores Mariano Puerta Manuel Sánchez Mariano ZabaletaThe following players received entry from the qualifying draw:
See main article: 2009 San Luis Potosí Challenger – Singles. Santiago Giraldo def. Paolo Lorenzi, 6–2, 6–7(3), 6–2
See main article: 2009 San Luis Potosí Challenger – Doubles. Santiago González / Horacio Zeballos def. Franco Ferreiro / Júlio Silva, 6–2, 7–6(5)
