| Year: | 2007 |
| Team: | Tuskegee Golden Tigers |
| Sport: | football |
| Conference: | Southern Intercollegiate Athletic Conference |
| Short Conf: | SIAC |
| Record: | 12–0 |
| Conf Record: | 7–0 |
| Head Coach: | Willie J. Slater |
| Hc Year: | 2nd |
| Stadium: | Abbott Memorial Alumni Stadium |
| Champion: | Black college national champion SIAC champion |
| Bowl: | Pioneer Bowl |
| Bowl Result: | W 58–51 vs. |
The 2007 Tuskegee Golden Tigers football team represented Tuskegee University as a member of the Southern Intercollegiate Athletic Conference (SIAC) during the 2007 NCAA Division II football season. Led by second-year head coach Willie J. Slater, the Golden Tigers compiled an overall record of 12–0, with a conference record of 7–0, and finished as SIAC co-champion. At the conclusion of the season, the Golden Tigers were also recognized as black college national champion.