| Sport: | football |
| Year: | 2001 |
| Team: | Wyoming Cowboys |
| Conference: | Mountain West Conference |
| Short Conf: | MW |
| Record: | 2–9 |
| Conf Record: | 1–6 |
| Hc Year: | 2nd |
| Off Coach: | Rusty Burns |
| Oc Year: | 2nd |
| Def Coach: | Matt Wallerstedt |
| Dc Year: | 2nd |
The 2001 Wyoming Cowboys football team represented the University of Wyoming in the 2001 NCAA Division I-A football season. The team's head coach was Vic Koenning, who was in his first year at Wyoming. They played their home games at War Memorial Stadium in Laramie, Wyoming, and competed in the Mountain West Conference.