| Year: | 2000 |
| Conference: | Missouri Valley Conference |
| Division: | I |
| Gender: | Men's |
| Teams: | 10 |
| Arena: | Kiel Center |
| City: | St. Louis, Missouri |
| Champions: | Creighton |
| Titlecount: | 6th |
| Coach: | Dana Altman |
| Coachcount: | 2nd |
| Mvp: | Ryan Sears |
| Mvpteam: | Creighton |
The 2000 Missouri Valley Conference men's basketball tournament was played after the conclusion of the 1999–2000 regular season at the Kiel Center in St. Louis, Missouri.
The Creighton Bluejays defeated the in the championship game, 57–45, and as a result won their 6th MVC Tournament title and earned an automatic bid to the 2000 NCAA tournament. Ryan Sears of Creighton was named the tournament MVP.