See main article: 2000 Generali Ladies Linz.
Mary Pierce was the defending champion,[1] but she did not compete this year due to injury.
Lindsay Davenport won the title, defeating Venus Williams in the final, 6–4, 3–6, 6–2. With the win, Davenport ended Williams' 35-match winning streak, extending from the Wimbledon Championships earlier in the year.
The top four seeds who played received a bye into the second round.