| Year: | 2000 |
| Team: | Colorado Buffaloes |
| Sport: | football |
| Conference: | Big 12 Conference |
| Division: | North Division |
| Short Conf: | Big 12 |
| Record: | 3–8 |
| Conf Record: | 3–5 |
| Head Coach: | Gary Barnett (2nd season) |
| Off Coach: | Shawn Watson (1st season) |
| Codef Coach1: | Tom McMahon |
| Codef Coach2: | Vince Okruch |
| Off Scheme: | Multiple |
| Def Scheme: | 4–3 multiple |
| Stadium: | Folsom Field |
The 2000 Colorado Buffaloes football team represented the University of Colorado at Boulder during the 2000 NCAA Division I-A football season. The team played their home games at Folsom Field in Boulder, Colorado. They participated in the Big 12 Conference in the North Division. They were coached by head coach Gary Barnett.
See also: 2000 NCAA Division I-A football rankings.