The qualifying rounds for the 2000–01 UEFA Champions League began on 12 July 2000. In total, there were three qualifying rounds which provided 16 clubs to join the group stage.
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| Qualify for the group stage | |
| Eliminated in the Third qualifying round; Advanced to the UEFA Cup first round |
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The draw for this round was performed on 23 June 2000 in Geneva, Switzerland.
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KR Reykjavík won 6–2 on aggregate.----Levski Sofia won 6–0 on aggregate.----2–2 on aggregate. Haka won on away goals.----Red Star Belgrade won 5–0 on aggregate.----Levadia Maardu won 6–2 on aggregate.----BATE Borisov won 3–2 on aggregate.----Shamkir won 5–3 on aggregate.----Shelbourne won 2–1 on aggregate.----Zimbru Chișinău won 6–4 on aggregate.----FBK Kaunas won 4–3 on aggregate.
The draw for this round was performed on 23 June 2000 in Geneva, Switzerland.
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Anderlecht won 4–2 on aggregate.----Beşiktaş won 2–1 on aggregate.----Brøndby won 3–1 on aggregate.----Polonia Warsaw won 7–4 on aggregate.----Rangers won 4–1 on aggregate.----Inter Bratislava won 1–0 on aggregate.----Helsingborgs IF won 3–0 on aggregate.----Red Star Belgrade won 4–2 on aggregate.----Shakhtar Donetsk won 9–2 on aggregate.----Slavia Prague won 5–1 on aggregate.----Rosenborg won 4–2 on aggregate.----Sturm Graz won 5–1 on aggregate.----Zimbru Chișinău won 2–1 on aggregate.----Dunaferr won 4–2 on aggregate.
The draw for this round was performed on 21 July 2000 in Nyon, Switzerland.
The losing teams advanced to the first round of the 2000–01 UEFA Cup.
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Valencia won 4–1 on aggregate.----Sparta Prague won 2–0 on aggregate.----Hamburger SV won 2–0 on aggregate.----Helsingborgs IF won 1–0 on aggregate.----Beşiktaş won 6–1 on aggregate.----Lyon won 4–2 on aggregate.----Anderlecht won 1–0 on aggregate.----Rangers won 6–0 on aggregate.----1–1 on aggregate. Dynamo Kyiv won on away goals.----Panathinaikos won 4–3 on aggregate.----Leeds United won 3–1 on aggregate.----Sturm Graz won 3–2 on aggregate.----Rosenborg won 4–3 on aggregate.----Galatasaray won 4–3 on aggregate.----Milan won 6–1 on aggregate.----Shakhtar Donetsk won 2–1 on aggregate.