The 2000–01 United Counties League season was the 94th in the history of the United Counties League, a football competition in England.
| Competition: | United Counties League Premier Division |
| Season: | 2000–01 |
| Winners: | Boston Town |
| Relegated: | Potton United Eynesbury Rovers |
| Matches: | 420 |
| Total Goals: | 1510 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The Premier Division featured 20 clubs which competed in the division last season, along with one new club:
| Competition: | United Counties League Division One |
| Season: | 2000–01 |
| Winners: | Daventry Town |
| Promoted: | Daventry Town Deeping Rangers |
| Matches: | 306 |
| Total Goals: | 1102 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
Division One featured 18 clubs which competed in the division last season, no new clubs joined the division this season.
Northampton Vanaid changed name to Northampton Sileby Rangers.