| Competition: | Slovenian Second League |
| Season: | 2000–01 |
| Winners: | Triglav Kranj |
| League Topscorer: | Borut Arlič (22 goals) |
| Total Goals: | 684 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Slovenian Second League season started on 13 August 2000 and ended on 10 June 2001. Each team played a total of 29 matches.