| Club: | Empoli |
| Season: | 2000–01 |
| Mgrtitle: | Head coach |
| Manager: | Silvio Baldini |
| Stadium: | Stadio Carlo Castellani |
| League: | Serie B |
| League Result: | 5th |
| Cup1: | Coppa Italia |
| Cup1 Result: | First round |
| League Topscorer: | Massimo Maccarone (16) |
| Season Topscorer: | Massimo Maccarone (16) |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 season was the 90th season in the history of Empoli F.C. and the club's second consecutive season in the second division of Italian football. In addition to the domestic league, Empoli participated in this season's edition of the Coppa Italia.
See main article: 2000–01 Serie B.
Source: [1]
See main article: 2000–01 Coppa Italia.
|-! colspan=14 style=background:#DCDCDC; text-align:center| Goalkeepers|-! colspan=14 style=background:#DCDCDC; text-align:center| Defenders|-! colspan=14 style=background:#DCDCDC; text-align:center| Midfielders|-! colspan=14 style=background:#DCDCDC; text-align:center| Forwards