| Season: | 1999–2000 |
| Nextseason: | 2000–01 |
1999–2000 Ukrainian First League was the ninth season of the Ukrainian First League which was won by FC Dynamo-2 Kyiv. The season started on July 25, 1999, and finished on June 19, 2000.
Two clubs promoted from the 1998-99 Ukrainian Second League.
One club was relegated from the 1998-99 Ukrainian Top League:
In 1999-00 season, the Ukrainian First League consists of the following teams:
Statistics are taken from here.[1]
| Scorer | Goals (Pen.) | Team | |
|---|---|---|---|
| 1 |  Pavlo Parshyn | 17 (5) | Polissya Zhytomyr |
| 2 | Oleh Venhlinskyi | 16 (1) | Dynamo-2 Kyiv |
| 3 | Myroslav Bundash | 15 (5) | Zakarpattia Uzhhorod |
| 4 | Vadym Plotnikov | 14 (3) | Stal Alchevsk |
| 5 | Oleh Hrytsay | 13 (2) | FC Cherkasy |
| 6 | Ovik Halstyan | 12 (3) | Torpedo / Chornomorets-2 |
| 7 | Viktor Arefyev | 11 (2) | Stal / CSKA-2 |
| Samir Hasanov | 11 (6) | Metalurh Nikopol | |
| 9 | Serhiy Babiychuk | 10 (6) | Yavir-Sumy |
| Vadym Chernyshenko | 10 (6) | Polihraftekhnika Oleksandriya |