The women's 100 metres hurdles event at the 1994 World Junior Championships in Athletics was held in Lisbon, Portugal, at Estádio Universitário de Lisboa on 21 and 22 July.
| Gold | Kirsten Bolm | |
| Silver | LaTasha Colander | |
| Bronze | Diane Allahgreen |
22 July
Wind: +0.5 m/s
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| align=left | align=left | 13.26 | |||||
| align=left | align=left | | 13.30 | ||||
| align=left | align=left | 13.31 | |||||
| 4 | align=left | align=left |  | 13.59 | |||
| 5 | align=left | align=left |  | 13.66 | |||
| 6 | align=left | align=left | | 13.72 | |||
| 7 | align=left | align=left |  | 13.74 | |||
| align=left | align=left |  | DNF |
21 July
Wind: +1.0 m/s
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 13.30 | Q | ||
| 2 | align=left | align=left | | 13.32 | Q | ||
| 3 | align=left | align=left | | 13.37 | Q | ||
| 4 | align=left | align=left | | 13.67 | Q | ||
| 5 | align=left | align=left |  | 13.70 | |||
| 6 | align=left | align=left |  | 13.74 | |||
| 7 | align=left | align=left | 14.10 | ||||
| align=left | align=left | DNF |
Wind: +1.1 m/s
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | 13.25 | Q | |||
| 2 | align=left | align=left | 13.48 | Q | |||
| 3 | align=left | align=left | | 13.65 | Q | ||
| 4 | align=left | align=left | | 13.67 | Q | ||
| 5 | align=left | align=left |  | 13.87 | |||
| 6 | align=left | align=left | 13.91 | ||||
| 7 | align=left | align=left |  | 13.98 | |||
| align=left | align=left |  | DNF |
21 July
Wind: +0.5 m/s
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 13.40 | Q | ||
| 2 | align=left | align=left | 13.48 | Q | |||
| 3 | align=left | align=left | 13.87 | Q | |||
| 4 | align=left | align=left | | 13.88 | q | ||
| 5 | align=left | align=left | 13.96 | ||||
| 6 | align=left | align=left |  | 13.98 | |||
| 7 | align=left | align=left | 14.01 | ||||
| 8 | align=left | align=left | | 14.82 |
Wind: +2.2 m/s
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | 13.46 w | Q | |||
| 2 | align=left | align=left | 13.87 w | Q | |||
| 3 | align=left | align=left | 13.94 w | Q | |||
| 4 | align=left | align=left |  | 14.10 w | |||
| 5 | align=left | align=left |  | 14.17 w | |||
| 6 | align=left | align=left |  | 14.55 w | |||
| 7 | align=left | align=left | | 17.94 w |
Wind: +0.4 m/s
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 13.29 | Q | ||
| 2 | align=left | align=left | | 13.63 | Q | ||
| 3 | align=left | align=left | | 13.70 | Q | ||
| 4 | align=left | align=left | 14.01 | ||||
| 5 | align=left | align=left | | 14.12 | |||
| 6 | align=left | align=left | 14.24 | ||||
| 7 | align=left | align=left |  | 14.28 | |||
| 8 | align=left | align=left | | 14.85 |
Wind: +2.9 m/s
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 13.35 w | Q | ||
| 2 | align=left | align=left | | 13.54 w | Q | ||
| 3 | align=left | align=left | | 13.60 w | Q | ||
| 4 | align=left | align=left | | 13.66 w | q | ||
| 5 | align=left | align=left | | 13.69 w | q | ||
| 6 | align=left | align=left | | 13.89 w | q | ||
| 7 | align=left | align=left |  | 13.96 w | |||
| 8 | align=left | align=left |  | 14.31 w |
According to an unofficial count, 31 athletes from 23 countries participated in the event.