| Competition: | Segunda División |
| Season: | 1965 |
| Winners: | Ferrobádminton |
| Promoted: | Ferrobádminton |
| Relegated: | None |
| Prevseason: | 1964 |
| Nextseason: | 1966 |
The 1965 Segunda División de Chile was the 14th season of the Segunda División de Chile.
Ferrobádminton was the tournament's champion.[1]