| Dates: | 3 April 1965 – 27 February 1966 |
| Winners: | Universidad de Chile (5th title) |
| Relegated: | Coquimbo Unido |
| Matches: | 306 |
| Total Goals: | 1027 |
| Continentalcup1: | 1966 Copa Libertadores |
| Continentalcup1 Qualifiers: | Universidad de Chile Universidad Católica |
| League Topscorer: | Héctor Scandolli (25) |
| Highest Attendance: | 74,522 |
| Attendance: | 3,046,357 |
| Average Attendance: | 9,955 |
| Prevseason: | 1964 |
| Nextseason: | 1966 |
The 1965 Campeonato Nacional de Fútbol Profesional, was the 33rd season of top-flight football in Chile. Universidad de Chile won their fifth title, also qualifying for the 1966 Copa Libertadores.[1]