| Season: | 1964–65 |
| Total Goals: | 809 |
| Average Goals: | 3.37 |
| Nextseason: | 1965–66 |
The Dutch Eerste Divisie in the 1964–65 season was contested by 16 teams. Willem II won the championship for the second time.
Promoted from the 1963–64 Tweede Divisie:
Relegated from the 1963–64 Eredivisie:
Willem II & Elinkwijk played a match to determine the league champions (on the field of BVV).