First-seeded Joan Hartigan defeated Mall Molesworth 6–1, 6–4, in the final to win the women's singles tennis title at the 1934 Australian Championships.
The seeded players are listed below. Joan Hartigan is the champion; others show the round in which they were eliminated.
Joan Hartigan (champion) Mall Molesworth (finalist) Emily Hood Westacott (quarterfinals) Nancy Chitty (first round) Nell Hall (quarterfinals) Louie Bickerton (semifinals) Kathrine Le Mesurier (semifinals) Ula Valkenburg (quarterfinals)