| National Challenge Cup | |
| Year: | 1927 |
| Country: | United States |
| Dates: | 20 February 1927 – 1 May 1927 |
| Defending Champions: | Bethlehem Steel |
| Winners: | Fall River F.C. |
| Count: | 2 |
| Second: | Holley Carburetor |
| Semifinal1: | Sparta ABA |
| Semifinal2: | Bethlehem Steel |
| Prev Season: | 1926 |
| Next Season: | 1928 |
The 1927 National Challenge Cup was the annual open cup held by the United States Football Association now known as the Lamar Hunt U.S. Open Cup.
The finalists played as follows:[1]
| align=right | Holley | align=center | Fall River | ||
| align=right | Dick | align=center | G | Tom Blair | |
| align=right | Boath | align=center | LB | Dave Gibson | |
| align=right | Hayston | align=center | RB | Charlie McGill | |
| align=right | Stewart | align=center | LHB | Hugh Coyle | |
| align=right | Dalrymple | align=center | CHB | ||
| align=right | Gallagher | align=center | RHB | Bill McPherson | |
| align=right | Love | align=center | OL | Dave McEachran | |
| align=right | Rutherford | align=center | IL | Jimmy Kelly | |
| align=right | Gerrit Visser | align=center | CF | Harold Brittan (C) | |
| align=right | Connally (C) | align=center | IR | James "Tec" White | |
| align=right | Forrester | align=center | OR | Dougie Campbell |