1-phosphofructokinase explained

1-phosphofructokinase should not be confused with Phosphofructokinase 1.

In enzymology, 1-phosphofructokinase is an enzyme that catalyzes the chemical reaction

ATP + D-fructose 1-phosphate → ADP + D-fructose 1,6-bisphosphate

Thus, the two substrates of this enzyme are ATP and D-fructose 1-phosphate, whereas its two products are ADP and D-fructose 1,6-bisphosphate. The enzyme was first described and characterized in the 1960s.^{[1] [2]}

This enzyme belongs to the phosphofructokinase B (PfkB) or Ribokinase family of sugar kinases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor.^{[3] [4]} The systematic name of this enzyme class is ATP:D-fructose-phosphate 6-phosphotransferase. Other names in common use include fructose-1-phosphate kinase, 1-phosphofructokinase (phosphorylating), D-fructose-1-phosphate kinase, fructose 1-phosphate kinase, and 1-phosphofructokinase. This enzyme participates in fructose and mannose metabolism. The members of the PfkB/RK family are identified by the presence of three conserved sequence motifs and their enzymatic activity generally shows a dependence on the presence of pentavalent ions.^[5]

Structure

As of 2021, two structures have been solved for this class of enzymes, with the PDB accession codes and, both from structural genomics efforts. The protein is a homodimer.

Notes and References

1. Reeves RE, Warren LG, Hsu DS . 1966 . 1-Phosphofructokinase from an anaerobe . J. Biol. Chem. . 241 . 1257 - 61 . 4222878 . 6 . 10.1016/S0021-9258(18)96768-2 . free .
2. Sapico V, Anderson RL . 1969 . D-fructose 1-phosphate kinase and D-fructose 6-phosphate kinase from Aerobacter aerogenes. A comparative study of regulatory properties . J. Biol. Chem. . 244 . 6280 - 8 . 4242639 . 22 . 10.1016/S0021-9258(18)63534-3 . free .
3. Park J, Gupta RS: Adenosine kinase and ribokinase--the RK family of proteins. Cell Mol Life Sci 2008, 65: 2875-2896.
4. Bork P, Sander C, Valencia A: Convergent evolution of similar enzymatic function on different protein folds: the hexokinase, ribokinase, and galactokinase families of sugar kinases. Protein Sci 1993, 2: 31-40.
5. Maj MC, Singh B, Gupta RS: Pentavalent ions dependency is a conserved property of adenosine kinase from diverse sources: identification of a novel motif implicated in phosphate and magnesium ion binding and substrate inhibition. Biochemistry 2002, 41: 4059-4069.